Evaluation of bacteriophage AV-08 for simultaneous biocontrol of Salmonella Montevideo and Escherichia coli O157:H7 in experimentally contaminated chicken skin
Posted: June 7th, 2012 - 4:11pm
Source: Journal of Food Safety
The aim of this research was to evaluate the efficacy of bacteriophage Av-08 for reducing Salmonella Montevideo and Escherichia coliO157:H7 presence from chicken skin surface at 4C. Significant reduction (P < 0.05) of S. Montevideo (5.16 log10 colony-forming units [cfu]/4 cm2 chicken skin) and E. coli O157:H7 (4.9 log10 cfu/4 cm2 chicken skin) was achieved. When phage was applied to the bacterial mixture, a reduction of 4.4 and 4.69 log10 cfu/4 cm2 of S. Montevideo and E. coli O157:H7 was obtained on the chicken skin surface, respectively. No significant reduction was observed by buffer application, compared with the control. Phage Av-08 was able to survive on the chicken skin surface at concentrations fluctuating from 7.27 to 8.44 log10 PFU/4 cm2 for 6 days. These results demonstrated the ability of phage Av-08 in reducing the presence of S. Montevideo and E. coli O157:H7 from chicken skin surface at 4C.
Recent antimicrobial and disinfectant bacterial resistance have renewed interest of using bacteriophage biocontrol against foodborne bacterial pathogens. In this research lytic bacteriophage Av-08 was evaluated for reducing Salmonella Montevideo and Escherichia coliO157:H7 from chicken skin surface. Actually, commercial bacteriophage preparations have been exploited for biocontroling foodborne bacterial pathogens. Phage Av-08 was able to simultaneously reduce S. Montevideo and E. coli O157:H7 presence from chicken skin. Therefore, bacteriophage Av-08 showed potential to be exploited for biocontroling Salmonella and E. coli O157:H7 from foods.