Incidence and genetic variability of Listeria species from three milk processing plants
Posted: May 19th, 2011 - 2:49pm
Source: Food Control
The presence of Listeria in three milk processing environments as a potential source of milk contamination was assessed. Swab samples (n=210) taken from milk processing plants were examined. Sample sites included the milk processing equipment, besides areas handling raw and pasteurised milk. The USDA Listeria-selective enrichment procedure was used to process the samples. Forty one (19.52%) Listeria isolates were recovered. The isolates were further subjected to biochemical and genotypic characterization. Out of 41 isolates, 16 (7.62%) were confirmed as Listeria monocytogenes, 2 (0.95%) as L. ivanovii, 19 (9.05%) as L. innocua. 1 (0.48 %) as L. seeligeri and 3 (1.43%) as L. grayi. All the L. monocytogenes isolates were positive for the hlyA gene. PCR based serotyping revealed all L. monocytogenes to be of 1/2a, 1/2c, 3a and 3c serovar group. AscI and ApaI restriction analysis yielded four PFGE clusters for 16 L. monocytogenes isolates obtained from raw milk collector, milk silos, buttermilk mixer, cheese and other milk product processor. No predominant PFGE cluster was observed among these L. monocytogenes isolates. The main sources of L. monocytogenes were found to be raw milk collector and milk silos. In the present study L. monocytogenes was isolated from milk and milk products processing plants which could cross-contaminate the processed products and may possess a potential threat to public health.
►We report the presence of Listeria in three milk processing environments as a potential source of milk contamination. ► The incidence of L. monocytogenes and L. ivanovii was found to be 7.62% and 0.95%. ► PCR based serotyping revealed all L. monocytogenes to be of 1/2a, 1/2c, 3a and 3c serovar group. ► PFGE analysis of the selected strains demonstrated variability.