Testing

  • Posted: August 23rd, 2012 - 9:35pm by Doug Powell

    There was this one time, Chapman came to Manhattan (Kansas) and lasted one quarter of a Kansas State football game before rushing home with explosive diarrhea.

    My whiny kid didn’t help either.

    He spent the rest of the visit holed up downstairs, sucking back Gatorade and sitting on the toilet.

    When he got back to North Carolina he had the wherewithal to donate a stool sample, and eventually found out he was part of a state-wide antibiotic-resistant campylobacter outbreak.

    In light of the German-based E. coli O104 outbreak in raw sprouts last year, researchers in Germany and Sweden are now calling for all stool samples from patients with diarrhea to be tested for enteropathic E. coli.

    Writing in Eurosurveillance, the authors state:

    Following an outbreak of enterohaemorrhagic Escherichia coli (EHEC) in Germany 2011, we observed increases in EHEC and non-EHEC E. coli cases in Bavaria. We compared the demographic, clinical and laboratory features of the cases reported during the outbreak period, but not related to the outbreak, to the cases reported before and after. The number of EHEC and non-EHEC E. coli cases notified per week during the outbreak was fivefold and twofold higher respectively, compared to previous years. EHEC cases notified during the outbreak were more often reported with bloody diarrhoea, and less often with unspecified diarrhoea, compared to the other periods. They were more often hospitalised during the outbreak and the following period compared to the period before. Their median age (26.5 years, range: 0–90) was higher compared to before (14.5 years, range: 0–94) and after (5 years, range: 0–81). The median age of non-EHEC E. coli cases notified during the outbreak period (18 years, range 0–88) was also higher than before and after (2 years, p<0.001). The surveillance system likely underestimates the incidence of both EHEC and non-EHEC E. coli cases, especially among adults, and overestimates the proportion of severe EHEC cases. Testing all stool samples from patients with diarrhoea for enteropathic E. coli should be considered.

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  • Posted: July 17th, 2012 - 6:46am by Doug Powell

    Policy requires tough choices, and rhetorical appeal.

    Accuracy also helps.

    According to an AP story, “the U.S. Agriculture Department grudgingly extended the life of the nation's largest produce-safety testing program on Monday, just as the initiative was slated to be shut down.”

    The largest produce testing programs are in industry. What’s more important is that the data be made public so taxpayers don’t have to pay what’s already been done.

    “The tiny program samples thousands of high-risk fruits and vegetables for pathogens each year, and has found more than two dozen bacteria-laced examples that prompted recalls of lettuce, tomatoes and other foods from grocery stores.”

    Seek and ye shall find. Did the program prevent any outbreaks? Probably not. That’s because preventing the bugs setting up shop is far more important, especially with fresh produce, than testing.

    As food safety czar Mike Taylor said the other day, there’s so much more that goes into the safety of produce.

     

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  • Posted: July 13th, 2012 - 4:10am by Doug Powell

    Where some see conspiracy, others see redundancy.

    The Washington Post reports Congress is poised to scrap funding for the Microbiological Data Program in the U.S. Department of Agriculture.

    USDA officials have suggested that the initiative would be a better fit for the Food and Drug Administration, which regulates vegetables and fruits. But that agency lacks the money needed to marshal more inspectors, and there’s no sign that the program will be moved there.

    Mike Taylor, the FDA’s deputy commissioner for foods, tells the Washington Post today there’s so much more that goes into the safety of produce.

    And that starts on the farm.

    Testing is a way to verify that food safety programs are working, but on its own, don’t mean much. There is lots of testing that goes on. Make it public, make the testing data part of the marketing of produce; market food safety.

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  • Posted: July 7th, 2012 - 2:53am by Doug Powell

    My friend Jim Romahn has been reporting on agriculture in Canada since federal Ag Minister Eugene Whelan started wearing green Stetsons.

    Jim used to write speeches for Gene.

    And he’s getting snarkier.

    I know the feeling.

    Jim writes the U.S. Department of Agriculture is adopting a new multi-testing system for meat that will make it much more difficult to sneak illegal residues into the country.

    But the Canadian Food Inspection Agency is, once again, lagging behind.

    When I asked if the CFIA is aware of the U.S. change and whether it's doing anything similar, I got a nifty dissembling spin-doctored response.

    Yes, the CFIA said, it's aware of the U.S. move.

    Yes, it said, it tests meat, poultry and eggs for more than 300 chemicals.

    And yes, it is using a multi-residue test.

    However, that multi-residue test is limited to about 30 antibiotics. That's nowhere close to what the U.S. is now doing.

    The single-sample testing the U.S. is implementing is for antibiotics, metals and growth promotants.

    In the past, meat could sneak by if the sample was tested for one chemical or for one type of residue, such as antimicrobials. Not now.

    When it comes to food safety and integrity, the CFIA just says Canada has the highest standards in the world, and one of the best inspection systems in the world. It's just hot air, folks.

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  • Posted: June 20th, 2012 - 7:57am by Doug Powell

    While the case count remains at 19 in the E. coli O157:H7 outbreak in Belgium linked to américain préparé containing raw hamburger, the slaughterhouse that supplied the beef has pledged to increase testing.

    Our French friend, Albert Amgar, wrote, it’s out of the ordinary to see a slaughterhouse communicate in such an open manner.

    The abattoir in Genk (Limbourg province) is going to undergo even stricter testing following an infection of Escherichia coli from which 19 people were stricken in Limbourg this weekend. According the Federal Agency for the Security of the Food Chain (AFSCA), it is now certain that the infection came from this abattoir. The management of the slaughterhouse confirmed on their part that it is not out of the question that the victims kept the filet américain (that made them sick) in a place that was not sufficiently cold. But it also admits that the infection could have come from within the slaughterhouse, in spite of strict hygiene tests.

    A French video can be seen at this link:

    http://www.deredactie.be/cm/vrtnieuws.francais/mediatheek_fr/2.3816/1.1334561

    Thanks to Albert for finding the article and video, and Amy for translating.

    Albert also had some sardonic words for the Belgian Minister of Agriculture, Sabine Laruelle, who attempted to reassure the public by reminding everyone that it was the common E. coli O157 strain and not the one that killed all the Germans last year.

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  • Posted: May 5th, 2012 - 7:17pm by Doug Powell

    Who knows how E. coli O157:H7 got into these berry munchy thingies, but kudos to the company for its internal testing which found the culprit and issued the voluntary recall.

    FunFresh Foods, Inc. of San Clemente, California is voluntarily recalling a single lot of its 6 ounce packages of FunFresh Foods™ World Berries™ Organic “Cacao Nibs” because they may be contaminated with Escherichia coli O157:H7

    Approximately 500 packages of affected product were distributed from April 12 through April 17, 2012 from this lot and as of the date of this release, 263 of these packages have already been retrieved from retail stores. Product was distributed to health and natural food retail stores located in the following states: AK, AR, AZ, CA, CT, FL, GA, ID, IL, IN, MI, MN, MO, MS, MT, NC, ND, NH, NJ, NM, OH, OK, OR, SC, SC, TN, TX, WA, WI, and WV.

    The product comes in a 6 oz pouch with the World Berries™ logo identified as Organic Cacao Nibs with the following UPC code 632474929022, affected lot code 161104 and the use by date for products for the affected lot 04/14, which are laser etched on the vertical edge of the back panel.

    No illnesses have been reported to date in connection with this product.

    The potential for contamination was identified through the company’s own audit testing of finished product which detected the presence of E. coli 0157:H7. Production and distribution of the product has been suspended while FDA and the company continue their investigation. No other lots of this product and no other FunFresh Foods products are affected by this recall.

    Consumers should not consume the product. Consumers who have purchased 6 ounce packages of "Cacao Nibs" are urged to return them to the place of purchase for a full refund. Alternatively, consumers can call the company which will arrange for a full refund and for retrieval of affected products. Consumers may contact the company between Monday through Friday, 8:00 a.m. and 5:00 p.m. Pacific Time at 1-800-232-8619.

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  • Posted: February 24th, 2012 - 10:02pm by Doug Powell

    Stigi et al. report in the March, 2012, issue of Emerging Infectious Disease that in a survey of laboratories in Washington State, increased use of Shiga toxin assays correlated with increased reported incidence of non-O157 Shiga toxin–producing Escherichia coli (STEC) infections during 2005–2010.

    Despite increased assay use, only half of processed stool specimens underwent Shiga toxin testing during 2010, suggesting substantial underdetection of non-O157 STEC infections.

    Strains of Shiga toxin (Stx)–producing Escherichia coli (STEC) are differentiated by the O antigen on their outer membrane and are broadly classified as O157 or non-O157 STEC. The ability to produce Stx is a key virulence trait of STEC. STEC infections in humans often cause a self-limited diarrheal illness but can be complicated by hemorrhagic colitis or hemolytic uremic syndrome.

    Unlike other E. coli strains, serogroup O157 isolates do not ferment sorbitol and are readily identified by culture, appearing colorless on sorbitol MacConkey agar. Both O157 and non-O157 STEC can be identified by detecting Stx with nonculture assays that became commercially available in the United States in 1995. The Centers for Disease Control and Prevention (CDC) published formal STEC testing recommendations for clinical laboratories in 2009, advocating that all stool specimens submitted for routine bacterial pathogen testing be simultaneously cultured for O157 STEC and tested with a nonculture assay to detect Stx. Use of this testing protocol ensures timely identification of all STEC infections. Exclusive testing for Stx delays specific identification of O157 STEC and may impede prompt detection of common-source outbreaks.

    Non-O157 STEC infection has been a nationally notifiable condition since 2000. Although studies have documented the increased incidence of reported non-O157 STEC infections over the past decade, few have determined the proportion of laboratories that routinely test all submitted stool specimens for Stx and, to our knowledge, no study has quantified STEC testing practices by wwwnc.cdc.gov/eid/article/18/3/11-1358_article.htmproportion of stool specimens processed for bacterial culture. Our objectives, therefore, were to quantify statewide STEC testing practice by proportion of stool specimens processed for bacterial culture and to determine the contribution of enhanced STEC testing practice to increased reported incidence of non-O157 STEC infections.

    The complete report is available at: wwwnc.cdc.gov/eid/article/18/3/11-1358_article.htm.

     

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  • Posted: February 8th, 2012 - 10:58pm by Doug Powell

    The U.S. Food Safety and Inspection Service announced on Feb. 8 it is extending the implementation date for routine sampling of six additional shiga-toxin producing E. coli serogroups (O26, O45, O103, O111, O121 and O145) for 90 days, according to the North American Meat Processors Association. The date was extended from March 5 to June 4.

    NAMP says the extension was granted to give extra time to establishments so they could validate their test methods and detect these pathogens prior to entering the commerce stream.

    Initially, FSIS plans to sample raw beef manufacturing trimmings and other raw ground beef product components both imported and produced domestically, plus test the serogroups’ samples.

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  • Posted: November 19th, 2011 - 3:14pm by Doug Powell

    Pamela Riemenschneider of The Packer writes that audits, testing and food safety programs are a part of daily life for any produce operation.

    In the Rio Grande Valley, companies work to foster a culture of food safety among their employees.

    “One of the challenges of a food safety program is to not treat it as if you’re studying for the test, but to accept it and embrace it as a way of doing business,” said Chris Eddy, general manager of Edinburg, Texas-based Frontera Produce Ltd.

    “That’s our focus, and we’re seeing a lot of success there and getting a buy-in from our employees.”

    That “it’s time for our annual audit, let’s do an extra sweep” attitude is long gone.

    The company is spreading this culture out to all of the sheds it operates and represents, Eddy said.

    Curtis DeBerry, president of Boerne, Texas-based Progreso Produce Ltd., said his company is rolling out in-house microbial testing in addition to its regular audits and Global Food Safety Initiative certification.

    “We’ve gone completely out on our own,” he said.

    “We’re doing the microbial testing in-house weekly. We’re going to step it up and be much more involved in the testing itself and the auditing in between, both in our facilities and out in the fields.”

    DeBerry said his company’s enhanced focus was driven by the buyer community and Progreso’s decision to enhance the program.

    At Bebo Distributing Inc. in Pharr, Texas, the packing lines are getting mechanical enhancements in the name of food safety.

    The company recently installed a new packing line that includes a chlorine wash.

    All this sounds great and shows how food safety requires numerous flexible and creative approaches. But why weren’t these firms and thousands of others actively enhancing the safety of fresh fruits and vegetables in the 1990s, when produce had clearly emerged as a significant source of foodborne illness?

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  • Posted: July 25th, 2011 - 8:15pm by Doug Powell

    hamburger.grind_.jpg

    Elizabeth Weise writes in the USA Today today or tomorrow that a growing chorus of lawmakers, food-safety and consumer advocates are is demanding the six other non-O157 shiga toxin producing E. coli strains – the Big 6 – be declared illegal in meat as E. coli O157:H7 was in 1994. And edited version of the story is below.

    In the absence of specific federal oversight, however, some companies have begun their own testing for these pathogens to protect consumers and their own bottom lines.

    First out of the chute was Costco, which began testing its ground beef two months ago. Beef Products Inc., the nation's largest supplier of lean beef, began testing on July 18.

    There's also movement in the produce and leafy greens world, where multiple producers and retailers have been testing for E. coli O157:H7 since the spinach outbreak that almost wiped out the leafy green vegetable market in 2006.

    In the past few months, newly available tests have made it possible to check for a broader number of the microbes and they now include the harmful group of E. coli strains beyond O157:H7 known as the Big Six.

    The reasons these bugs aren't currently regulated are a mix of politics, money and plain biology — the bacteria are constantly evolving and turning up new and nastier forms, making writing rules about them a bit of a nightmare.

    For example, the German E. coli variant that sickened more than 4,075 in Europe and killed 50, including one Arizona resident who traveled to Germany, wasn't known before this spring (and is not part of the Big 6).

    As it stands now, any meat that tests positive for the O157:H7 form of E. coli has to be removed from the market. But for other types of E. coli that are known to harm humans, it takes an illness to trigger a recall, says Nancy Donley, of STOP Foodborne Illness, a food-safety advocacy group started by parents who've lost children to these pathogens. "This is clearly not as it should be," she says.

    The push to get these debilitating but non-O157:H7 forms of E. coli regulated has been coming for a long time. The Centers for Disease Control and Prevention has long required they be reported.

    But as tests become available, some companies aren't waiting for the feds to act. In the last six months, test kits for leafy greens have become available for the Big Six E. coli variants from IEH Laboratories in Lake Forest Park, Wash.; DuPont Qualicon in Wilmington Del.; and BioControl Systems in Bellevue, Wash.; and others are in the works. For ground beef, they're in late testing phase or became available in the past two months. In just the past two weeks, tests for the German E. coli O104:H4 variant hit the market.

    IEH Laboratories has been testing for a broad range of these pathogenic E. colis for years now. "We had been finding a lot of these things in products right and left," says President Mansour Samadpour.

    A table of non- E. coli O157 STEC outbreaks is available at http://bites.ksu.edu/nonO157outbreaks
     

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